Equipment

Electron Microscopes

Serial Block-Face Electron Microscopy Field Emission ZEISS Sigma Variable pressure with 3View technology from Gatan. Technology for exceptional imaging and analysis. It is possible to examine large sections from different tissues; view imaging is possible with image size of up to 32k by 32k pixels. In combination with 3View ultramicrotome, allows for high resolution, 3D imaging of tissues and even whole organisms, such as Drosophila. In the shortest possible time.

Location: O&N4, 7th floor in room 07.438.

JEOL JSM 7200F LV Scanning Electron Microscope. Our new field emission scanning microscope utilizes JEOL proprietary in-lens Schottky Plus technology that allows improvement in the resolution at low accelerating voltages (1.6 nm @ 1 kV), and achieves a maximum probe current of 300 nA.

Location: O&N4, 7th floor in room 07.434.

The SECOM platform from Delmic, NL. The SECOM is a platform that integrates fluorescence and scanning electron microscopy in ons device by equipping a scanning electron microscope (JEOL JSM 7200F LV) with an inverted fluorescence microscope. SECOM is a unique integrated solution for correlative light and electron microscopy that enables to do correlative microscopy extremely fast with the highest optical quality and overlay accuracy.

Location: O&N4, 7th floor in room 07.434.

JEOL JEM2100-LaB6 Transmission Electron Microscope can be operated at a range of acceleration voltages of 40-200kV and features and Oxford INCA Energy Dispersive X-ray Spectroscopy (EOS) system and a Gatan Tridiem Electron Energy Loss Spectroscopy (EELS) filter/spectrometer for element mapping and image filtering. Additionally, the instrument is equipped with a tomography recording unit using a 70°/70° goniometer to create 3D-images from its samples and TSEM detectors (bright- and darkfield). Used for cryo- and RT imaging.

Location: O&N1, 6th floor in room 06.654.

JEOL JEM1400-LaB6 Transmission Electron Microscope is the working horse of the Electron Microscopy Platform. With the large field of view Olympus Quemesa 11Mpx camera it provides high-contrast micrographs of conventionally prepared or high-pressure frozen and freeze-substituted biological specimens.

Location: O&N4, 7th floor in room 07.434.

Sample preparation equipment

Standard sample preparation

  • Leica/Reichert Ultracut E
  • Leica Ultracut UCT
  • Leica Ultracut EM UC7
  • Leica Ultracut S
  • RMC Powertome PC Ultramicrotome
  • Leica EM ACE 600 High Vacuum Coater
  • Leica EM CPD300 Critical Point Drier
  • Leica EM KMR2 Glass Knife Maker
  • Leica/Reichert Glass Knife Maker
  • Leica IC80 HD Live Video Camera

Location: O&N4 7th floor.

Cryo sample preparation

  • Leica EM PACT2 High Pressure Freezeing Apparatus
  • Leica EM AFS2 Automatic Freeze substitution system with EM FSP
  • Leica Ultracut EM UC7 with Leica FC7Cryo chamber and Leica Cryo-Spher
  • Leica Cryo chamber for Leica Ultracut UCT
  • EMBO Plunge freezer
  • EDWARDS T-Station 75 - turbo pumping station for Cryo-TEM
  • Gatan Transfer Station for 914 High Tilt Cryo Holder
  • Gatan Transfer Station for Cryo Holder
  • Gatan Samrt Set Cold Stage Controller 900

Location: O&N1, 6th floor in room 06.654

Software

  • HCS analysis
  • 3D software: Imaris, Arivis, Amira (FEI), Dragonfly Pro 2.0 (Zeiss)
  • Digital Micrograph Software (Gatan)
  • IMOD
  • ImageJ/Fiji support
  • TEMography package

Location: O&N4, 7th floor in room 07.404

X