Education channel

Welcome to our EDUCATION CHANNEL!

We will be adding here some useful information, protocols, manuals, links to different websites, etc., to gain more knowledge in the electron microscopy world.

 

  • Analysing EM data; these softwares can help!

semi-automated analysis of 3D-EM datasets:

Segmentation of EM images:

Alignment of multidimensional images:

3D reconstructions of macromolecular complexes:

 

  • We have a new Software for our JEOL TEM1400!

Click here to open our quick manual!

 

 

  • What about the pixelsize of your EM images?

The pixelsize is depending on magnification and camera, Look in this table which pixelsize belongs to which magnification for a Olympus Quemesa camera.

  • How do cells and their organelles look like?

find a EM image of your structure of interest : 'Electron Microscopy Atlas of cells, tissues and organs'
find LM and EM images of your structure of interest: 'Histology Atlas'

  • How to prepare samples for EM?

Get an idea of the procedure for EM preparations by clicking: Protocol database

  • Hazards associated with EM Preparation!

Know your H- and R-phrases when you are using EM preparation chemicals! You can find them in the manufacturer's safety data sheets. Always work safely!
Here we have listed some hazard symbols which go for EM preparation chemicals:

Paraformaldehyde 32% solution, EM grade 
Glutaraldehyde 25% solution, EM g
rade
Osmium tetroxide solution
Sodium cacodylate trihydrate
Uranyl acetate

Paraformaldehyde 32% solution, EM grade
Glutaraldehyde 25% solution, EM grade
Osmium tetroxide solution

Paraformaldehyde 32% solution, EM grade 
Glutaraldehyde 25% solution, EM g
rade
Lead nitrate
Sodium cacodylate trihydrate
Uranyl acetate

Paraformaldehyde 32% solution, EM grade 
Glutaraldehyde 25% solution, EM g
rade
Lead nitrate
Osmium tetroxide solution
Uranyl acetate

  • Want to know more about EM?

TEM tutorials for interactive image classification, segmentation and analysis

EM for dummies

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