Single cell & microfluidics

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The single-cell analytics and microfluidics expertise unit at our center offers a wide and growing range of applications for single cell research, including single-cell transcriptomics, epigenomics, and proteomics.

It is a joint endeavor between the VIB-KU Leuven Center for Brain & Disease research and the VIB-KU Leuven Center for Cancer Biology, and is part of the Genomics Core at KU Leuven, working in close collaboration with the Lab of Reproductive Genomics (Thierry Voet lab).

SINGLE-CELL ANALYTICS

Dropseq

  • Microfluidics emulsion platform
  • Processing capability: ~1000–10,000 cells per chip
  • Downstream processing: single-cell data analytics

10X Chromium - scRNA seq

  • High throughput sc-RNAseq work flow – Profiling tissue constituents; population level profiling
  • Microfluidics emulsion platform – 8 samples/lanes can be processed in parallel
  • Processing capability - ~1000–10,000 cells per lane
  • 2–3 times higher sensitivity

SMARTseq - in collaboration with Genomics core (KU Leuven/UZ Leuven)

  • Plate based method (96 cells)
  • Ideal for profiling limited cell counts – rare cellular subfractions.
  • Complete gene profiling – provide information on the gene modification, eg. Splicing variants

Single cell ATAC seq

  • single-cell assay for transposase-accessible chromatin (scATAC-seq)
  • Assay for assessing Genome wide DNA accessibility – Identification of genetic regulatory elements
  • Plate based / Microfluidic assay (~100 cells)
  • Tn5 transposase, chops and tags the regulatory regions with sequencing adaptor

MICROFLUIDICS

Microfluidics is an integral part of high-dimensional single cell analytics and it is increasingly prevalent in Biology and Medicine.

Tech development

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