The single-cell analytics and microfluidics expertise unit at our center offers a wide and growing range of applications for single cell research, including single-cell transcriptomics, epigenomics, and proteomics.
It is a joint endeavor between the VIB-KU Leuven Center for Brain & Disease research and the VIB-KU Leuven Center for Cancer Biology, and is part of the Genomics Core at KU Leuven, working in close collaboration with the Lab of Reproductive Genomics (Thierry Voet lab).
SINGLE-CELL ANALYTICS
Dropseq
- Microfluidics emulsion platform
- Processing capability: ~1000–10,000 cells per chip
- Downstream processing: single-cell data analytics
10X Chromium - scRNA seq
- High throughput sc-RNAseq work flow – Profiling tissue constituents; population level profiling
- Microfluidics emulsion platform – 8 samples/lanes can be processed in parallel
- Processing capability - ~1000–10,000 cells per lane
- 2–3 times higher sensitivity
SMARTseq - in collaboration with Genomics core (KU Leuven/UZ Leuven)
- Plate based method (96 cells)
- Ideal for profiling limited cell counts – rare cellular subfractions.
- Complete gene profiling – provide information on the gene modification, eg. Splicing variants
Single cell ATAC seq
- single-cell assay for transposase-accessible chromatin (scATAC-seq)
- Assay for assessing Genome wide DNA accessibility – Identification of genetic regulatory elements
- Plate based / Microfluidic assay (~100 cells)
- Tn5 transposase, chops and tags the regulatory regions with sequencing adaptor
MICROFLUIDICS
Microfluidics is an integral part of high-dimensional single cell analytics and it is increasingly prevalent in Biology and Medicine.
Tech development
